Crystalline defects in polymer films significantly affect their structural integrity and performance. In this study, we investigate the lamellar morphology of uniaxially stretched poly(glycolic acid) (PGA) cast films using small-angle X-ray scattering (SAXS). For the first time, two-dimensional chord distribution functions (CDF) and correlation functions K(r) were applied to compare crystalline (fisheye defect) and non-crystalline regions. These functions were derived from 2D Fourier transforms of the weighted scattering intensity I(sāā, sā), followed by image refinement to enhance structural contrast. The results reveal higher lamellar density, thickness, and ordering in defect areas, attributed to faster crystallization during casting. Wide-angle X-ray scattering (WAXS) further confirms enhanced crystallinity at defect sites. This SAXS-based approach offers a powerful tool for resolving anisotropic microstructural features and provides new insights into defect formation mechanisms in polymer films.