In this study, hydrogels based on chia seed (Salvia hispanica L.) mucilage were prepared. Subsequent modification of the hydrogels with active compounds (gallic acid and amoxicillin) rendered the resulting hydrogels as antioxidant and antimicrobial biomaterials, respectively. The incorporation of the active compounds was investigated using Fourier-Transform Infrared Spectroscopy (FTIR). The equilibrium swelling ratios revealed that the hydrogels have swelled approximately >100 times their original weight. Surface morphological and elemental analysis of the prepared hydrogels was carried out using Scanning Electron Microscopy- Energy Dispersive X-Ray spectroscopy (SEM-EDX). Using the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay, the produced hydrogels' antioxidant potentials were demonstrated to be >80% effective. This inhibition also increased with the amount of gallic acid added to the hydrogels. Moreover, the amoxicillin-containing hydrogels have shown excellent antimicrobial properties against the Gram-negative bacterium, Escherichia coli. Collectively, the results presented herein demonstrate that naturally prepared hydrogels can be utilized to develop biomaterials with excellent antioxidant and antimicrobial properties for various biomedical applications.